(I) The quantification of fluorescence signal from IVIS images, n= 3. (H) Representative IVIS images from day 1 to day 14 after fluorochrome-labeled MSCs injection. (G) Representative IVIS images of heat pouch loaded with fluorochrome labeled MSCs from day 1 to day 14. (F) Quantification of mCherry signal from heart tissue after heart pouch implantation from Day 1 to Day 3 (n = 5). Immunofluorescence images of heart tissue after treatment on (D) Day 1 and (E) Day 3. Numbers indicate the percentage of cells in the respective quadrant. (C) Flow cytometry analysis of recipient co-cultured with labelling-MSCs. Labeling MSCs were stained with DiO before co-culture. (B) Confocal image of the co-cultured cells. MSCs were engineered to express mCherry-fusion protein (labeling MSCs). (A) Plasmid map for pcPPT-mPGK-attR-sLPmCherry-WPRE vector used for the labelling system. The origami structure allowed minimally invasive delivery of the pouch to the heart with two small incisions and can be refilled with the therapeutic of choice. In summary, we developed a fluid-driven heart pouch with a memory-shaped microfabricated lattice structure inspired by origami. The pouch's semi-permeable membrane successfully protected delivered cells from their surroundings, maintaining their viability while releasing paracrine factors to the infarcted site for cardiac repair. We tested the pouch's ability to deliver mesenchymal stem cells (MSCs) in a rodent model of acute myocardial infarction and demonstrated the feasibility of minimally invasive delivery in a swine model. The origami structure allowed minimally invasive delivery of the pouch to the heart with two small incisions and can be refilled multiple times with the therapeutic of choice.
We developed a fluid-driven heart pouch with a memory-shaped microfabricated lattice structure inspired by origami. Repeated dosing of therapeutics is difficult due to the invasive nature of such procedures. I used mine as a subtle Valentine’s Day decoration in my kitchen.Cardiac repair after heart injury remains a big challenge and current drug delivery to the heart is suboptimal. They’d be a darling way to give Valentine’s Day goodies to a friend.
Then your cute basket is ready to fill and hang and enjoy. Step 19: Once all of the folded edges and flap pieces are glued down, your basket will look smooth on the inside. Glue the handle in place and glue the side flaps shut. Step 17: Insert your ribbon or twine or rope handle into the area on each side of the hearts that overlaps. Step 16: Your heart basket is almost done! If you look inside, you’ll notice there are a lot of loose parts sticking out. Step 15: Glue the other side flaps of the hearts together the same way. Step 14: Open up the side flaps of the heart and overlap them so that the tip of one heart lines up with the tip of the other heart and the side flap edges also line up. You’ll notice the hearts have a smooth, pretty front side and a back side that has lots of flaps. Step 13: Repeat steps 1-12 to make a second paper heart. Lift up the tip of the heart, then tuck those two flaps inside and glue the two layers of the heart tip together. Step 12: Fold those two flaps up to be even with the bottom point of the heart. Step 11: Flip the paper over to the other side and you’ll see the heart shape with two extra flaps below it. Step 10: Fold the flat left edge inward and line it up with the center line. Step 9: Fold the top square shaped flaps inward to form triangle shapes. Open these slightly, then fold them open to make a triangle shape. Step 8: There are two small flaps along the top. Step 7: Flip the paper over and turn it 180 degrees. Fold the bottom point up to be even with the top of the center fold line. Step 6: Flip the paper over to the other side. Fold the bottom right corner in to the center line. Step 5: Fold the bottom left corner up and in to the center line. Step 4: Flip the paper over to the other side, with the skinny folded section on the bottom. Step 3: Fold the right side edge inwards until it lines up with the center crease line. Step 2: Fold the paper in half, then open it and fold it in half the other way, leaving T-shaped creases in the paper. I made three hearts in three different sizes and started with 12″ 14″ and 16″ squares. Step 1: Cut two squares of paper any size you want. Paper Squares ( Kraft Paper Roll, Wrapping Paper, Paper Grocery Bag).
Follow along and pause it after each step. It’s pretty simple to do and I’ve tried my best to break down every step clearly, but the video tutorial is going to be your best friend.
#ORIGAMI HEART HOW TO#
Today I’m going to show you how to make an origami paper heart basket. I just can’t believe all the cool things you can make just by folding paper. Learn how to fold a heart shaped basket out of paper.
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